| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gretna Elite Academy GEA Pre-ECNL-RL 09G 2 |
02077-0011G2U-0035 | Teresa DeGeorge |
| A2 | Heartland FC HFC Hurricanes |
05085-0011G2U-0006 | Scott Dubbs |
| A3 | Sporting Nebraska FC SOFC LINCOLN ELITE 09 |
03036-001ELG2U-2096 | Krista Kester |
| A4 | z-Infinite Sports World ISW Eclipse |
03124-0011G2U-0007 | Jeff Oksman |
| A5 | Sporting Nebraska FC SOFC G GOLD 09 |
03036-001OSG2U-2082 | John DeSalvo |
| A6 | Council Bluffs Soccer Club CBSC 2009G |
0O001-0011G2U-0005 | Shane Hoss |
| A7 | Bennington Soccer Club BENSC INFERNO (12G) |
03057-0011G2U-0026 | Trenton Vakiner |
| A8 | z-South Sioux City Soccer Club SSC Academy 09 - G12U |
04056-0012G2U-0002 | Thomas Otten |
| A9 | Eagles | NA | Nate Bettes |
| A10 | Gretna Elite Academy GEA Gold 10G |
02077-0011G1U-0018 | Raphael Martinez |